Publication details

New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.

Authors

PEKAROVÁ Michaela LOJEK Antonín MARTIŠKOVÁ Hana VAŠÍČEK Ondřej BINÓ Lucia KLINKE Anna LAU Denise KUCHTA Radek KADLEC Jaroslav VRBA Radimír KUBALA Lukáš

Year of publication 2011
Type Article in Periodical
Magazine / Source The Scientific World Journal
MU Faculty or unit

Faculty of Science

Citation
Web http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246759/?tool=pubmed
Doi http://dx.doi.org/10.1100/2011/321979
Field Ecology
Keywords Macrophages; L-arginine; inducible nitric oxide synthase; superoxide anion; NO
Description Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models. However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized. Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation. Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.