New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.

• Autoři: PEKAROVÁ Michaela; LOJEK Antonín; MARTIŠKOVÁ Hana; VAŠÍČEK Ondřej; BINÓ Lucia; KLINKE Anna; LAU Denise; KUCHTA Radek; KADLEC Jaroslav; VRBA Radimír; KUBALA Lukáš
• Rok publikování: 2011
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: The Scientific World Journal
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• www: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246759/?tool=pubmed
• Doi: http://dx.doi.org/10.1100/2011/321979
• Obor: Ekologie - společenstva
• Klíčová slova: Macrophages; L-arginine; inducible nitric oxide synthase; superoxide anion; NO
• Popis: Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models. However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized. Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation. Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.