Publication details
ELECTROCHEMICAL STUDY OF INTERACTION OF 23 FRAGMENTS OF METALLOTHIONEIN WITH CISPLATIN – HIGH THROUGHPUT METHOD
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Proceedings |
| Conference | XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků. |
| MU Faculty or unit | |
| Citation | |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | metallothionein;protein;zinc;cadmium;cisplatine;electrochemistry |
| Description | Metallothionein is metal binding protein occurring intracellularly in animals. It can bind essential zinc ions and also toxic metals like cadmium. In cancer research the problem of resistance of cancer cells against anticancer drugs was described. Metallothionein was found to be one of cause of the resistance due to its ability to bind toxic metals. In this study we determined the influence of aminoacid sequence in metalothionein on to affinity to cisplatin using electrochemical method. For high throughput automatic analysis we designed automated flow injection analysis with electrochemical detection, which was carried out by Coulochem III as amperometric detector. |