Publication details

Electrophoretic techniques in the analysis of polydeoxycytidylic acids

Authors

BITTOVÁ Miroslava JELÍNKOVÁ Kateřina ROBLOVÁ Vendula TRNKOVÁ Libuše

Year of publication 2012
Type Article in Proceedings
Conference XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords electrophoresis oligonucleotides coated capillary
Description Electrophoretic techniques such as capillary zone electrophoresis and one-dimensional polyacrylamide gel electrophoresis were applied in the separation of polydeoxycytidylic acids between 3 and 18 nucleotides in chain length (dC3-dC18). In capillary zone electrophoresis (CZE), the electrophoretic behavior of homo-oligonucleotides in hydrophilic coated capillary (CEP) and polyethyleneimine coated capillary (PEI) was followed under various experimental conditions. One-dimensional polyacrylamide gel electrophoresis was chosen as a supplemental method in the characterization of electrophoretic properties of polydeoxycytidylic acids.
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