Project information
The role of cytoskeleton and cell wall in the cell polarization
- Project Identification
- GA204/99/D025
- Project Period
- 1/1999 - 1/2002
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Medicine
The role of cytoskeleton (actin, microtubules and membrane skeleton) in the process of the cell polarity will be studied on the model of budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces japonicus var. versatilis. Mating process, protoplasts regeneration and osmotic stress will be studied as processes changing cell polarity. Cdc mutants showing defect in the cell polarization (cdc24,42), mutant in Kar 3p controlling karyogamy, and mutants in high osmolarity glycerol pat hway (hog1 and pbs2) as well as microtubule and actin inhibitors will serve as an experimental approaches. Fluorescence microscope techniques, including indirect immunofluorescence, and electron microscope techniques will be applied in the study of chang es in cytoskeleton and cell structure.
Publications
Total number of publications: 12
2003
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Immunodetection of Spectrin-like Proteins in Yeasts
Canadian Journal of Microbiology, year: 2003, volume: 49, edition: 3
2002
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Detection of spectrin-like protein in yeasts: spectrin-actin relations
Sborník X. Cytoskeletálního klubu, year: 2002
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SEM and AquaSEM studies of S. cerevisiae and C. moggii colonies
Sborník XXXth Annual conference on yeasts, year: 2002
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Spectrin-like protein in yeasts
Sborník XXX th Annual Conference on yeasts, year: 2002
2001
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Cytoskeleton in regenerating protoplasts and restoration of cell polarity in the yeast Saccharomyces cerevisiae
Acta Biologica Hungarica, year: 2001, volume: 52, edition: 2
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Interaction of benzo[c]phenanthridine and protoberberine alkaloids with animal and yeast cells
Cell Biology and Toxicology, year: 2001, volume: 17, edition: 1
2000
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Cell wall and cytoskeleton reorganization as the response to hyperosmotic shock in Saccharomyces cerevisiae
Archives of Microbiology, year: 2000, volume: 173, edition: 4
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Cytoskelet a mitochondrie u Xantophyllomyces dendrorhous (Phaffia rhodozyma)
Year: 2000, number of pages: 1 s.
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Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)
Folia Microbiologica, year: 2000, volume: 45, edition: 1
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Osmotic shock affects cell wall and cytoskeleton in Saccharomyces cerevisiae
Cellular and Molecular Biology, year: 2000, volume: 46, edition: -