Publication details
Cytoskeleton and mitochondrial topology in Xantophyllomyces dendrorhous (Phaffia rhodozyma)
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Periodical |
| Magazine / Source | Folia Microbiologica |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | cytoskeleton; Xanthophyllomyces; Phaffia rhodozyma; microtubules; mitochondria |
| Description | The morphology of budding and conjugating cells and associated changes in microtubules and actin distribution were studied in the yeast Phaffia rhodozyma (Basidiomycetes) by phase-contrast and fluorescence microscopy. The non-budding interphase cell showed a nucleus situated in the central position and bundles of cytoplasmic microtubules either stretching parallel to the longitudinal cell axis or randomly distributed in the cell; none of these, however, were in contact with the nucleus. During mitosis, the nucleus divided in the daughter cell, cytoplasmic microtubules disappeared and were replaced by a spindle. The cytoplasmic microtubules reappeared after mitosis had finished. Actin patches were present in both the bud and the mother cell. Cells were induced to mate by nitrogen depletion. The partner cells fused at their conjugation projections where actin patches had been accumulated . Cell fusion resulted in a zygote that produced basidiophore with parallel bundles of microtubules extended along the basidiophore axis and with actin patches concentrated at the cell poles. The fused nucleus moved towards the basidiophore tip. During this movement, meiotic division was taking place; the nuclei were eventually distributed to basidiospores. Mitochondria visualised by means of DIOC6 (3) appeared as vesicles of various sizes; their large amounts were found, often lying adjacent to microtubules, in the cytoplasm of both vegetative cells and zygotes. |
| Related projects: |