Project information
Activity of acidophilic sulfur-oxidizing bacteria under aerobic and anaerobic conditions
- Project Identification
- GA525/08/0697
- Project Period
- 1/2008 - 5/2012
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Science
- doc. Ing. Martin Mandl, CSc.
- doc. Mgr. Pavel Bouchal, Ph.D.
- doc. RNDr. Oldřich Janiczek, CSc.
- Keywords
- sulfur-oxidizing bacteria, acid mine drainage, sulfide wastes
The project proposal investigates activity of sulfur-oxidizing bacteria to obtain new results on process parameters which results in environmental acidification and toxic metal mobilization in sulfide wastes and abandoned mines. An Acidithiobacillus ferrooxidans mine isolate will be a model bacterium to study elemental sulfur oxidation. To learn more about the cell energetic profile, a method for ATP determination will be applied in relation to oxidation kinetics and assessment of an actual metabolic potential of bacteria to form sulfuric acid. The aerobic bacteria will be adapted to anaerobic sulfur oxidation to study process parameters under different physiological state of bacteria. Proteome analysis of aerobic and anaerobic bacteria will be performed to obtain knowledge on characteristic proteins which are formed during elemental sulfur oxidation and a cell switch to iron oxidation. This will provide a complex view to the metabolic control. The project can contribute to the study of physiology of sulfur-oxidizing bacteria in relation to their role in the environment.
Publications
Total number of publications: 23
2013
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Cellular ATP and biomass of attached and planktonic sulfur-oxidizing Acidithiobacillus ferrooxidans
Process Biochemistry, year: 2013, volume: 48, edition: 11, DOI
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Cellular ATP changes in Acidithiobacillus ferrooxidans cultures oxidizing ferrous iron and elemental sulfur
Geomicrobiology Journal, year: 2013, volume: 30, edition: 1, DOI
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Cellular ATP of iron-and sulfur-oxidizing bacteria as an indicator of cell energetics and growth
Year: 2013, type: Conference abstract
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Differences in cellular ATP levels in Acidithiobacillus ferrooxidans oxidizing ferrous iron and elemental sulfur
Year: 2013, type: Conference abstract
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Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis
Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, year: 2013, volume: 103, edition: 4, DOI
2012
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ATP assay for monitoring metabolic activity of acidophilic bacteria
Year: 2012, type: Conference abstract
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ATP measurements in acidophilic bacteria oxidizing iron(II) and elemental sulfur
Year: 2012, type: Conference abstract
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Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, year: 2012, volume: 101, edition: 3, DOI
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Reaction modelling of anaerobic elemental sulfur oxidation in biomining bacteria
Year: 2012, type: Conference abstract
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Stoichiometry of bacterial anaerobic oxidation of elemental sulfur by ferric iron
Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, year: 2012, volume: 101, edition: 4, DOI