Project information

Posttranslational modification of Dishevelled

Project Identification
KJB501630801
Project Period
1/2008 - 12/2010
Investor / Pogramme / Project type
Academy of Sciences of the Czech Republic
MU Faculty or unit
Faculty of Science
Keywords
Dishevelled; Wnt; posttranslational modification

Wnts are secreted factors and regulators of embryonic development, disease and regeneration. Mutations in the Wnt pathway are crucial in pathogenesis of many diseases, including cancer. Wnts bind Frizzled family of receptors and activate several downstream signalling pathways. The immediate downstream component necessary for transduction of all branches of Wnt signalling is a scaffolding protein Dishevelled (Dvl). In the present project, we aim to understand the biochemical nature of Wnt-dependent activation Dvl by analyzing and mapping in detail the physiologically relevant posttranslational modifications of Dvl. We plan to use several approaches of protein biochemistry, proteomics and site-directed mutagenesis to uncover and validate critical phosphorylations and other possible posttranslational modifications. We expect that our findings will increase our understanding of Wnt signal transduction and provide a basis for therapeutic interventions of Wnt-associated diseases.

Publications

Total number of publications: 4