Project information
Regulation and manipulation of plant meiosis
- Project Identification
- CZ.02.1.01/0.0/0.0/15_003/0000479 (kod CEP: EF15_003/0000479)
- Project Period
- 1/2017 - 10/2022
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Operational Programme Research, Development and Education
- Priority axis 1: Strengthening capacities for high-quality research
- MU Faculty or unit
- Central European Institute of Technology
Meiosis lies at the heart of sexual reproduction, the most successful reproductive strategy creating new genetic combinations every generation. This project aims at understanding the mechanisms that define meiotic division and gamete differentiation in plants; part of the effort will be establishing technologies allowing in vitro differentiation of male gametes, live imaging of meiosis and the manipulation of meiotic processes. The approaches will include microfluidics for in vitro cultivation of reproductive tissues coupled with time-laps microscopy (anther on chip technology), next generation sequencing and bioinformatics for identification of new meiotic regulators, CRISPR/Cas9 genome editing and development of techniques for the delivery of biologically active compounds into meiotic. This project well complements ongoing research activities at CEITEC and will strengthen its excellence at multiple levels: 1) It brings unique technology and instrumentation, which will yield important and highly visible discoveries. 2) It combines different expertise and contributes to the multidisciplinarity of the research centre. 3) It has applied potential in plant breeding. 4) It will facilitate establishing close ties to the Vienna Biocenter and promote cross-border collaboration with this prestigious research cluster.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 16
2020
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Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters
Plant Cell, year: 2020, volume: 32, edition: 5, DOI
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Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy
elife, year: 2020, volume: 9, edition: FEB, DOI
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Nonsense-Mediated RNA Decay Factor UPF1 Is Critical for Posttranscriptional and Translational Gene Regulation in Arabidopsis
Plant Cell, year: 2020, volume: 32, edition: 9, DOI
2019
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artMAP: A user-friendly tool for mapping ethyl methanesulfonate-induced mutations in Arabidopsis
PLANT DIRECT, year: 2019, volume: 3, edition: 6, DOI
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Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants
LIFE SCIENCE ALLIANCE, year: 2019, volume: 2, edition: 3, DOI
2018
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Functional Characterization of SMG7 Paralogs in Arabidopsis thaliana
Frontiers in Plant Science, year: 2018, volume: 9, edition: NOV, DOI