Retinal Organoids: Exploring the Effects of Photo-Stimulation on Human Retina
- Project Identification
- Project Period
- 1/2021 - 12/2023
- Investor / Pogramme / Project type
- Czech Science Foundation
- MU Faculty or unit
- Faculty of Medicine
Pluripotent stem cell-derived retinal organoids represent a reliable experimental retinal model, and also potential source for cell replacement therapy for treating retinal dystrophies. However, in vitro generated retinal cells are often poorly differentiated with impaired functionality demonstrated by not fully developed photoreceptor outer segments and less synaptic connectivity, which can be contributed to the lack of natural instructive signals that is normally available in vivo. In this project we aim to address these limitations by introducing instructive signals using photo-stimulation of human retinal cells. We have developed a unique optical device for high-throughput multi-wavelength light illumination of retinal organoids. Our preliminary results clearly indicate that photo-stimulation of differentiating retinal cells leads to enhanced differentiation. The proposed strategy combines the innovative photo-stimulation approach with stem cell biology research, allowing to study photo-induced effects on retinal development from a uniquely different perspective.
Sustainable Development Goals
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Total number of publications: 2