Informace o projektu
Retinal Organoids: Exploring the Effects of Photo-Stimulation on Human Retina
- Kód projektu
- GA21-08182S
- Období řešení
- 1/2021 - 12/2023
- Investor / Programový rámec / typ projektu
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Grantová agentura ČR
- Standardní projekty
- Fakulta / Pracoviště MU
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Lékařská fakulta
- Mgr. Tomáš Bárta, Ph.D.
- Canan Çeliker, MSc
- Mgr. Eva Hrubá, Ph.D.
- Tereza Křivská
- Bc. Ondřej Macura
- Bc. Barbora Sedláčková
- Mgr. Kamila Weissová, Ph.D.
Pluripotent stem cell-derived retinal organoids represent a reliable experimental retinal model, and also potential source for cell replacement therapy for treating retinal dystrophies. However, in vitro generated retinal cells are often poorly differentiated with impaired functionality demonstrated by not fully developed photoreceptor outer segments and less synaptic connectivity, which can be contributed to the lack of natural instructive signals that is normally available in vivo. In this project we aim to address these limitations by introducing instructive signals using photo-stimulation of human retinal cells. We have developed a unique optical device for high-throughput multi-wavelength light illumination of retinal organoids. Our preliminary results clearly indicate that photo-stimulation of differentiating retinal cells leads to enhanced differentiation. The proposed strategy combines the innovative photo-stimulation approach with stem cell biology research, allowing to study photo-induced effects on retinal development from a uniquely different perspective.
Cíle udržitelného rozvoje
Masarykova univerzita se hlásí k cílům udržitelného rozvoje OSN, jejichž záměrem je do roku 2030 zlepšit podmínky a kvalitu života na naší planetě.
Publikace
Počet publikací: 3
2023
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Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light
iSCIENCE, rok: 2023, ročník: 26, vydání: 7, DOI
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MIR204 n.37C>T variant as a cause of chorioretinal dystrophy variably associated with iris coloboma, early-onset cataracts and congenital glaucoma
Clinical Genetics, rok: 2023, ročník: 104, vydání: 4, DOI
2022
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LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters
Life science alliance, rok: 2022, ročník: 5, vydání: 8, DOI