Project information

Project information
Structural basis for transcription termination of nonpolyadenylated transcripts

Project Identification
Project Period
1/2009 - 12/2011
Investor / Pogramme / Project type
Academy of Sciences of the Czech Republic
MU Faculty or unit
Central European Institute of Technology
Structure; RNA recognition; transcription termination; protein-RNA complex; nuclear magnetic resonance; RNA quality control; RNA processing

RNA polymerases produce RNA transcripts by initiating and terminating transcription in response to specific sequence elements. Signals that terminate transcription reside either in the DNA template sequence or, alternatively, in the nascent RNA transcript. RNA Polymerase II transcribes not only mRNA but also a subset of small nuclear and small nucleoar RNAs, and a class of intergenic and anti-sense RNAs. Termination of these transcripts requires the Nrd1 complex which recognizes specific sequence signals in RNA transcript by unknown mechanism. Here, we propose to use multidimensional NMR spectroscopy to investigate the structural basis for RNA recognition by the Nrd1 complex and to reveal the structural determinants that define the spacing and orientation of termination elements for efficient nonpoly(A) termination.


Total number of publications: 10

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