Publication details

Využití DNA čipové technologie ve výzkumu genetické podmíněnosti schizofrenie

Title in English The use of DNA microarray technology in research of genetic cause of schizophrenia
Authors

LOCHMAN Jan ŠERÝ Omar

Year of publication 2013
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description Fast development of microarray techniques in recent years has allowed us to perform genotyping of large number of single nucleotide polymorphisms (SNPs) as a part of GWAS (genome-wide association studies). In our study we have observing SNPs associated with a risk of developing schizophrenia. We have designed DNA microarray analyzing 96 SNPs using GoldenGate method on BeadExpress reader (Illumina). In the selection we have followed the results of recent GWAS (Genome Wide Association Studies) and those from our previous studies. The whole analysis comprised 405 men samples from patients and healthy controls. The avarage call rate in our samples was 98%, while in further statistic analysis we have used only those samples with call rate above 90%. After analysis using Fisher two-tailed test a Bonferroni corrections we have found six polymorphisms (rs6595788, rs28705343, rs3737967, rs77692880, rs9960767 a rs6104567) significantly associated with a risk of developing schizophrenia (p < 0.05). Interestingly, from 12 most significanly associated SNPs from GWAS analyses there have been found association only in four SNPs (rs6595788, rs28705343, rs3737967 a rs9960767). This confirms the necessity to replicate GWAS results by conducting smaller unbiased studies with better specified individuals and to consider involvment of gender on the degree of these association.
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