Publication details
Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
| Authors | |
|---|---|
| Year of publication | 2014 |
| Type | Article in Periodical |
| Magazine / Source | Analytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Web | http://pubs.acs.org/doi/abs/10.1021/ac402823n |
| Doi | http://dx.doi.org/10.1021/ac402823n |
| Field | Analytic chemistry |
| Keywords | Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput |
| Description | This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser. |
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