Publication details

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

Authors

BEDNAŘÍK Antonín KUBA Pavel MOSKOVETS Eugene TOMALOVÁ Iva KRÁSENSKÝ Pavel HOUŠKA Pavel PREISLER Jan

Year of publication 2014
Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Central European Institute of Technology

Citation
Web http://pubs.acs.org/doi/abs/10.1021/ac402823n
Doi http://dx.doi.org/10.1021/ac402823n
Field Analytic chemistry
Keywords Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput
Description This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info