On Proper Simulation of Phenomena Influencing Image Formation in Fluorescence Microscopy
|Year of publication
|Article in Proceedings
|2014 IEEE International Conference on Image Processing
|MU Faculty or unit
|Use of computers, robotics and its application
|Simulation; Biomedical imaging; Fluorescence microscopy; Photobleaching; Uneven illumination
|The simulation plays an important role in biomedical image analysis as it can inherently provide large collections of image data with absolute ground truth. Compared to real image data typically annotated by some expert, the computer generated data still lacks the authenticity due to the simplifications of various natural phenomena. In this paper, we focus on simulating the photobleaching effect and the uneven illumination, being simplified or even omitted from the majority of present simulation toolkits, to considerably improve the authenticity of computer generated data.