Publication details

Haematopoietic developmental potential of human pluripotent stem cell lines

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Year of publication 2014
Type Article in Periodical
Magazine / Source Folia biologica
MU Faculty or unit

Faculty of Informatics

Field Genetics and molecular biology
Keywords human pluripotent stem cells; hematopoietic development; embryoid bodies; CD34+ cells
Description The generation of hematopoietic progenitors from human pluripotent stem cells (hPSCs) presents great promise for cell-replacement therapies. However, current protocols for hematopoietic differentiation of hPSCs suffer from low efficiency and functional defects in the derived cells. The technology is also limited by variable ability of hPSC lines to generate blood cells in vitro. To address this issue, methodologies for hematopoietic differentiation in feeder-free conditions were applied to available human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines in this study. It was found that these cell lines did not generate hematopoietic progenitors in such an extent as H1 and H9 hESC lines used for this purpose in the vast majority of relevant studies. These results suggest that for clinical application of blood cells derived from hPSCs, possibly from autologous hiPSCs, it is necessary to overcome the variability in the hematopoietic developmental potential of individual hPSC lines.
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