Publication details

Electrochemical Analysis of Sybr Green I and its Interaction with DNA

Authors

DUDOVÁ Zdenka PIVOŇKOVÁ Hana HAVRAN Luděk FOJTA Miroslav

Year of publication 2015
Type Article in Periodical
Magazine / Source XXXV MODERNI ELEKTROCHEMICKE METODY
Citation
Keywords Sybr Green I; Pyrolytic graphite electrode; Square wave voltammetry; PCR
Description There are used many methods such real-time PCR and electrophoresis in molecular biology and biochemistry which are based on utilization of a fluorescent dye Sybr Green I (SG) for selective detection of double stranded (ds) DNA and its quantification. SG is a planar molecule (see Fig. 1) that intercalates into the DNA double helix and simultaneously can bind into minor groove of dsDNA. In our work we focused on electrochemical behavior of SG on a pyrolytic graphite electrode (PGE) using square-wave voltammetry (SWV). The voltammetric method was used in measurements of SG interactions with DNA at the PGE.

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