Informace o publikaci

Electrochemical Analysis of Sybr Green I and its Interaction with DNA

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DUDOVÁ Zdenka PIVOŇKOVÁ Hana HAVRAN Luděk FOJTA Miroslav

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj XXXV MODERNI ELEKTROCHEMICKE METODY
Citace
Klíčová slova Sybr Green I; Pyrolytic graphite electrode; Square wave voltammetry; PCR
Popis There are used many methods such real-time PCR and electrophoresis in molecular biology and biochemistry which are based on utilization of a fluorescent dye Sybr Green I (SG) for selective detection of double stranded (ds) DNA and its quantification. SG is a planar molecule (see Fig. 1) that intercalates into the DNA double helix and simultaneously can bind into minor groove of dsDNA. In our work we focused on electrochemical behavior of SG on a pyrolytic graphite electrode (PGE) using square-wave voltammetry (SWV). The voltammetric method was used in measurements of SG interactions with DNA at the PGE.