Publication details

Močové mikroRNA jako potenciální biomarkery karcinomu močového měchýře

Title in English Urinary microRNAs as potential biomarkers of bladder cancer
Authors

JURÁČEK Jaroslav STANÍK Michal PELTANOVÁ Barbora ADAMCOVÁ Soňa DOLEŽEL Jan FEDORKO Michal RADOVÁ Lenka SLABÝ Ondřej

Year of publication 2018
Type Article in Proceedings
Conference Klinická Onkologie
MU Faculty or unit

Central European Institute of Technology

Citation
Keywords urinary microRNAs; biomarkers; bladder cancer
Description Background: Currently, there are no urinary-based tumour markers with sufficient sensitivity and specificity to replace cystoscopy in the detection of bladder cancer (BCA). Urinary microRNAs are emerging as clinically useful class of biomarkers for early and non-invasive detection of urologic malignancies. Patients and Methods: In this study, 155 patients with BCA and 83 healthy controls were enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays and candidate miRNAs further validated in independent cohort using specific TaqMan assays and qRT-PCR method. Results: Whole-genome profiling identified miRNA signature with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the independent validation phase of the study, three miRNAs were confirmed to have significantly higher levels in urine of patients with BCA in comparison with control groups (P < 0.0001). In addition, we observed significant decrease in two miRNAs (P < 0.01) concentrations in the urinary samples collected 3 months after surgery compared to pre-operative samples. Conclusion: We identified and validated miRNAs to have significantly higher concentrations in urine of patients with BCA in comparison with controls. Our data have shown that urinary microRNAs could serve as sensitive and specific biomarkers enabling non-invasive detection of BCa.
Related projects: