Publication details

Tobacco BY-2 cell suspension as a model for assessment of pharmaceuticals phytotoxicity



Year of publication 2019
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description Pharmaceuticals and pharmaceutically active substances nowadays represent an important class of emerging environmental pollutants. Growth and developmental changes in plants induced by pharmaceuticals reflect changes of processes at the cellular and subcellular levels. Due to their growth and cellular characteristics, plant cell suspension cultures like Nicotiana tabacum BY-2 can be a suitable model for assessing toxicity. Non-steroidal anti-inflammatory drug diclofenac (DCF) belongs to currently monitored compounds in the environment because of its frequent occurrence and high potential toxicity. This study suggests that exposure to environmentally relevant concentrations of DFC (1, 10, and 100 ug/L) affected significantly the viability of BY-2 cells, suspension density and mitotic index. The growth and viability changes after 120 h of treatment were accompanied by increased production of total reactive oxygen species (ROS) and mitochondrial superoxide, and by down-regulation of reactive nitrogen species and total thiols in correlation to DCF treatment. DCF-induced oxidative stress triggered apoptotic alterations in BY-2 cells leading to programmed cell death – irregular or stretched nuclei were observed in up to 50% of cells. The response of the tobacco BY-2 cell suspension to DCF treatment is prompt and sensitive and selected parameters are suitable for toxicity evaluation.
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