Publication details

Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry

Authors

FARKA Zdeněk MICKERT Matthias Jürgen MIKUŠOVÁ Zuzana HLAVÁČEK Antonín BOUCHALOVÁ Pavla XU Wenshu BOUCHAL Pavel SKLÁDAL Petr GORRIS Hans-Heiner

Year of publication 2020
Type Article in Periodical
Magazine / Source Nanoscale
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://pubs.rsc.org/en/content/articlelanding/2020/nr/c9nr10568a
Doi http://dx.doi.org/10.1039/C9NR10568A
Keywords photon-upconversion nanoparticle; immunocytochemistry; immunocytochemistry; bioconjugation; surface modification; streptavidin
Description Immunohistochemistry (IHC) and immunocytochemistry (ICC) are routinely employed for the microscopic identification and diagnosis of cancerous cells in histological tissues and cell cultures. The maximally attainable contrast of conventional histological staining techniques, however, is low. While the anti-Stokes emission of photon-upconversion nanoparticles (UCNP) can efficiently eliminate optical background interference, excluding non-specific interactions of the label with the histological sample is equally important for specific immunolabeling. To address both requirements, we have designed and characterized several UCNP-based nanoconjugates as labels for the highly specific detection of the cancer biomarker HER2 on various breast cancer cell lines. An optimized streptavidin-PEG-neridronate-UCNP conjugate provided an unsurpassed signal-to-background ratio of 319, which was 50-fold better than conventional fluorescent labeling under the same experimental conditions. In combination, the absence of optical interference and non-specific binding lays the foundation for computer-based data evaluation in digital pathology.
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