Publication details

Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments

Authors

KUSHKEVYCH Ivan ABDULINA Daryna KOVÁČ Jozef DORDEVIĆ Dani VÍTĚZOVÁ Monika IUTYNSKA Galyna RITTMANN Simon K.-M. R.

Year of publication 2020
Type Article in Periodical
Magazine / Source Biomolecules
MU Faculty or unit

Faculty of Science

Citation
Web https://www.mdpi.com/2218-273X/10/6/921
Doi http://dx.doi.org/10.3390/biom10060921
Keywords hydrogen sulfide; toxicity; sulfate-reducing bacteria; cell-free extracts; ecotopes
Description A comparative study of the kinetic characteristics (specific activity, initial and maximum rate, and affinity for substrates) of key enzymes of assimilatory sulfate reduction (APS reductase and dissimilatory sulfite reductase) in cell-free extracts of sulphate-reducing bacteria (SRB) from various biotopes was performed. The material for the study represented different strains of SRB from various ecotopes. Microbiological (isolation and cultivation), biochemical (free cell extract preparation) and chemical (enzyme activity determination) methods served in defining kinetic characteristics of SRB enzymes. The determined affinity data for substrates (i.e., sulfite) were 10 times higher for SRB strains isolated from environmental (soil) ecotopes than for strains from the human intestine.
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