Publication details

Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells.

Authors

KOCHEROVA I. STEFANSKA K. BRYL R. PEREK J. PIEŃKOWSKI W. ŽÁKOVÁ Jana CRHA Igor VENTRUBA Pavel MOZDZIAK P. JEŠETA Michal

Year of publication 2020
Type Article in Periodical
Magazine / Source Medical Journal of Cell Biology
MU Faculty or unit

Faculty of Medicine

Citation
Web https://content.sciendo.com/view/journals/acb/8/4/article-p176.xml
Doi http://dx.doi.org/10.2478/acb-2020-0023
Keywords apoptosis-related genes; granulosa cells; primary culture
Description Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.

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