Informace o publikaci

Apoptosis related genes expression in primary in vitro culture of human ovarian granulosa cells.

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KOCHEROVA I. STEFANSKA K. BRYL R. PEREK J. PIEŃKOWSKI W. ŽÁKOVÁ Jana CRHA Igor VENTRUBA Pavel MOZDZIAK P. JEŠETA Michal

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Medical Journal of Cell Biology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://content.sciendo.com/view/journals/acb/8/4/article-p176.xml
Doi http://dx.doi.org/10.2478/acb-2020-0023
Klíčová slova apoptosis-related genes; granulosa cells; primary culture
Popis Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.

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