Publication details

Structural Characterization of the Interaction Between BRCA1-BARD1 and RNA Polymerase II



Year of publication 2020
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Description Transcrption is considered one of the major threats for genome stability; as the conflicts of the transcription machinery with the replication fork, or another barrier, can lead to double-stranded DNA breaks. Although maintaining genome integrity is crucial for the cell viability, the mechanisms responsible for avoiding these conflicts are poorly characterized. Therefore, my research project focuses on structural characterization of the interaction between RNA polymerase II (RNAPII) and BRCA1-BARD1 complex, one of possible players involved in maintaining the genome stability. Recently, we have confirmed the interaction between the C-terminal domain of RNAPII and the BRCT domains of BRCA1 and BARD1, respectively, and we are reconstituting the full-length complex for cryoelectron microscopy studies. Structural characterization of the complex, as well as description of the conditions under which it is formed will help us to analyse its function in preventing transcription-borne DNA damage. This, in turn, will help us to understand how cells coordinate transcription and other competing processes on DNA, such as replication or DNA repair.
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