Thermostable FGF2 polypeptide, use thereof

• Authors: DVOŘÁK Petr; KREJČÍ Pavel; BÁLEK Lukáš; EISELLEOVÁ Lívia; KONEČNÁ Žaneta; DVOŘÁK Pavel; BEDNÁŘ David; BREZOVSKÝ Jan; ŠEBESTOVÁ Eva; CHALOUPKOVÁ Radka; ŠTĚPÁNKOVÁ Veronika; VAŇÁČEK Pavel; PROKOP Zbyněk; DAMBORSKÝ Jiří; BOSÁKOVÁ Michaela
• Year of publication: 2023
• Type: Patent
• MU Faculty or unit: Faculty of Science
• Publisher: Canadian Intellectual Property Office
• State: Canada
• Patent's number: CA3006388
• web: https://brevets-patents.ic.gc.ca/opic-cipo/cpd/eng/patent/3006388/summary.html
• Description: The invention provides an isolated thermostable polypeptide possessing FGF2 activity and having at least 85% sequence identity to SEQ ID NO: 2 (FGF2 wt) or a functional fragment thereof, and comprising at least one amino acid substitution R31 L and the use thereof in the cell biology research, regenerative medicine and related medical applications or cosmetics. Further it discloses a culture medium comprising subjected FGF2 suitable for culturing a human pluripotent stem cells involving both human embryonic stem cells and induced pluripotent stem cells.