Publication details
Identifikace patogenů potravinového řetězce v přítomnosti inhibitorů PCR
| Title in English | Identification of food chain pathogens in the presence of PCR inhibitors |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | V. Pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | food chain; pathogens; PCR; inhibitors |
| Description | Different ways of PCR ready DNA matrix preparation from contaminated foods were tested. It was shown that Ca2+ ions inluenced PCR identification of bacteria in milk samples.Inhibitors of PCR of protein nature were proved in dried eggs. They can be eliminated using proteinase K and by boiling. Bacteria of genus Salmonella and Campylobacter contain PCR inhibitors. |
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