Publication details

In vivo imaging of cell interior using automated high-resolution cytometry

Authors

VAŘECHA Miroslav AMRICHOVÁ Jana ONDŘEJ Vladan LUKÁŠOVÁ Emilie KOZUBEK Stanislav MATULA Pavel MATULA Petr KOZUBEK Michal

Year of publication 2004
Type Article in Proceedings
Conference Biophysics of the Genome
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords living cell
Description The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on the study of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding fusion mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins.
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