Publication details

Different nuclear localization of aberrant and normal chromosome 8 homologues in intact or NaBt differentiated HT-29 cells



Year of publication 2004
Type Article in Proceedings
Conference Biophysics of the Genome
MU Faculty or unit

Faculty of Informatics

Field Genetics and molecular biology
Keywords cytometry
Description An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes. 2D analysis of the distances between centre of nucleus-to-centre of chromosome 8 territory revealed that sodium butyrate did not affect nuclear arrangem.
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