Publication details

Automated acquisition and processing of multidimensional image data in confocal in vivo microscopy

Authors

KOZUBEK Michal MATULA Petr MATULA Pavel KOZUBEK Stanislav

Year of publication 2004
Type Article in Periodical
Magazine / Source Microscopy research and technique
MU Faculty or unit

Faculty of Informatics

Citation
Field Use of computers, robotics and its application
Keywords automated microscopy; confocal microscopy; in vivo imaging; Nipkow disk
Description The successful development of visualization techniques for live cell imaging leads to the development of suitable software for the acquisition and processing of multidimensional image data. This report compares several possible approaches to image acquisition and processing in confocal in vivo microscopy and suggests new alternatives to the published methods. Special attention is paid to spinning disk systems based either on a classical Nipkow disk or on the microlens principle. This study shows how to optimize image acquisition process in live cell studies using camera binning feature and how to perform object tracking using a new fast image registration method based on the graph theory.
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