Publication details

A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples

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Year of publication 2007
Type Article in Periodical
Magazine / Source Acta Chiropterologica
MU Faculty or unit

Faculty of Science

Field Zoology
Keywords Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling
Description The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.
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