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A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples

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Název česky Rychlý test na druhovou identifikaci dvou kryptických netopýrů Pipistrellus pipistrellus a P. pygmaeus na základě PCR metody a jeho využití na muzejních vzorcích a vzorcích trusu
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KAŇUCH Peter HÁJKOVÁ Petra ŘEHÁK Zdeněk BRYJA Josef

Rok publikování 2007
Druh Článek v odborném periodiku
Časopis / Zdroj Acta Chiropterologica
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Zoologie
Klíčová slova Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling
Popis The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.
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