Publication details

Study of cryptogein recognition in plant defense reaction.

Authors

LOCHMAN Jan KAŠPAROVSKÝ Tomáš HORŇÁKOVÁ Darina SVOZILOVÁ Zuzana MIKEŠ Vladimír

Year of publication 2007
Type Article in Proceedings
Conference FEBS Journal
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords recognition tobacco cryptogein
Description Cryptogein is proteinaceus elicitor isolated from Phytophthora cryptogea able to trigger a plant defense reaction. We prepared a series of cryptogein mutants for study of interaction cryptogein - receptor. Cryptogein mutants were tested for ability to induce plant defense early events, i.e. synthesis of active oxygen species, pH changes and NO production, in suspension tobacco cells. Moreover interaction between cryptogein or cryptogein mutants and receptor was studied by piezoelectric biosensor. We focused on study of cryptogein mutants with low affinity to tobacco receptor. These mutants showed high threshold concentration in triggering production of active oxygen species, pH changes and NO production. However sensitivity of defense reactions was different between the mutants. Low affinity of cryptogein mutants to receptor indicate that mutation affect the binding site of cryptogein. Further, by piezoelectric biosensors was characterized interaction of cryptogein and tobacco receptor. This method is going to be used for study of cryptogein mutants with low elicitation activity and detail characterization of cryptogein binding site.
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