Publication details

Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC



Year of publication 2008
Type Article in Periodical
Magazine / Source Journal of Agricultural and Food Chemistry
MU Faculty or unit

Faculty of Science

Field Analytic chemistry
Keywords Ergosterol; Malt; Barley; Deoxynivalenol; Gushing; HPLC
Description In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program) and applied to 124 samples of barley and malt. Ergosterol was isolated by extraction, saponification and the quantification was performed using HPLC with diode array detection (DAD). The content of ergosterol ranged between LOD-36.3 mg/kg in barley and LOD-131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.
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