Publication details

Microtubules and actin cytoskeleton of potentially pathogenic basidimycetous yeast as target for antifungals

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Authors

KOPECKÁ Marie GABRIEL Miroslav

Year of publication 2009
Type Article in Periodical
Magazine / Source CHEMOTHERAPY
MU Faculty or unit

Faculty of Medicine

Citation
Web
Field Microbiology, virology
Keywords Yeast;Actin;Microtubules;Paclitaxel;Vincristine;Vinblastine;Methyl benzimidazole2yl carbamate;Thiabendazole; Cytochalasins A B D; Latrunculin A
Description The cytoskeleton was investigated as a potential target for the inhibition of cell division in Fellomyces fuzhou-ensis related to Cryptococcus neoformans.Vincristine, vinblastine,paclitaxel,methyl benzimidazole-2-yl carbamate (BCM),thiabendazole,cytochalasins A,B,D and latrunculin A were added to cells and investigated.Vincristine, vinblastine, paclitaxel, cytochalasins A,B and D transiently blocked proliferation. BCM disrupted microtubules,inhibited mitosis, but F-actin structures persisted and neck-less conidia originated. Latrunculin disrupted F-actin, cells became spherical,stalks and necks degenerated, microtubules persisted,mitosis,cytokinesis and conidiogenesis were blocked.The combined application of latrunculin and BCM disrupted F-actin and microtubules,inhibited cells became spherical and did not divide.Conclusion: Microtubules and F-actin are effective targets for permanent inhibition of nuclear and cell division and conidiogenesis by BCM and latrunculin A.
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