Growth/differentiation factor-15 inhibits differentiation into osteoclasts - A novel factor involved in control of osteoclast differentiation

• Authors: VAŇHARA Petr; LINCOVÁ Eva; KOZUBÍK Alois; JURDIC Pierre; SOUČEK Karel; ŠMARDA Jan
• Year of publication: 2009
• Type: Article in Periodical
• Magazine / Source: Differentiation
• MU Faculty or unit: Faculty of Science
• Field: Oncology and hematology
• Keywords: GDF-15; osteoclast; LNCaP; RAW264.7; prostate cancer
• Description: We show that both pure GDF-15 and the GDF-15-containing growth medium of 1,25(OH)2-vitamin D3-treated prostate adenocarcinoma LNCaP cells suppress formation of mature osteoclasts differentiated from RAW264.7 macrophages and bone-marrow precursors by MCSF/RANKL in dose-dependent manner. GDF-15 inhibits expression of c-Fos and activity of NFkB by delayed degradation of IkB. Moreover, GDF-15 inhibits expression of carbonic anhydrase II and cathepsin K, key osteoclast enzymes, and induces changes in SMAD and p38 signaling.

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