Expression of RAN, ZHX-2, and CHC1L genes in multiple myeloma patients and in myeloma cell lines treated with HDAC and Dnmts inhibitors.
|Year of publication||2010|
|Type||Article in Periodical|
|Magazine / Source||Neoplasma|
|MU Faculty or unit|
|Field||Oncology and hematology|
|Keywords||multiple myeloma; real time PCR; MOLP-8; RAN; ZHX-2; CHC1L|
|Description||Real time PCR is a powerful tool for studying the expression of genes involved in the pathophysiology of human diseases. Here, we described a detailed Real-Time PCR method for the detection of RAN, ZHX-2, and CHC1L expression, which could be applied in clinical situations. The expression profiles of these genes were studied in peripheral blood lymphocytes of healthy individuals, patients suffering from MM, and in the myeloma cell line, MOLP-8. Our experiments illustrate new methodological approaches and troubleshooting for conducting gene expression studies in clinical laboratories.|