Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

Publication details

Authors	HUSKA Dalibor ADAM Vojtěch BABULA Petr TRNKOVÁ Libuše HUBÁLEK Jaromír ZEHNÁLEK Josef HAVEL Ladislav KIZEK René
Year of publication	2011
Type	Peer-reviewed scientific article
Magazine / Source	Microchim. Acta
MU Faculty or unit	Faculty of Science
Citation
web	https://link.springer.com/article/10.1007/s00604-011-0545-z
Doi	https://doi.org/10.1007/s00604-011-0545-z
Field	Physical chemistry and theoretical chemistry
Keywords	Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles
Description	The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)