Informace o publikaci

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

Autoři

HUSKA Dalibor ADAM Vojtěch BABULA Petr TRNKOVÁ Libuše HUBÁLEK Jaromír ZEHNÁLEK Josef HAVEL Ladislav KIZEK René

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Microchim. Acta
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://link.springer.com/article/10.1007/s00604-011-0545-z
Doi http://dx.doi.org/10.1007/s00604-011-0545-z
Obor Fyzikální chemie a teoretická chemie
Klíčová slova Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles
Popis The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info