Publication details


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ŠTEFL Richard SKLENÁŘ Vladimír

Year of publication 2012
Type Chapter of a book
MU Faculty or unit

Central European Institute of Technology

Description The chapter addresses the current methodologies for RNA investigation by liquid-state NMR spectroscopy. The current technology for the preparation and purification of uniformly and segmentally isotopically labeled RNA is reviewed. An overview of essential spectroscopic strategies for NMR studies of RNA is presented. Critical steps in RNA investigations by NMR are demonstrated by protocols, examples, along with practical advice for troubleshooting.
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