Project information
Epigenetic control of gene expression in malignant diseases
- Project Identification
- NC6987
- Project Period
- 1/2002 - 12/2004
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- R&D of Oncology
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- DNA-microarrays;cancer;chromosome structure;epigenetic regulation;cell differentiation
- Cooperating Organization
-
Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
- Responsible person MUDr. Zdeněk Kořístek, Ph.D.
- Responsible person prof. MUDr. Jan Žaloudík, CSc.
Using DNA-microarray technology, the transcirptome maps will be determined for several normal and tumor cell populations in different stages of progression. Comparison ot maps should allow us to find out combinations of genes related to cancerogenesis. The structure of chromosomes will be inbestigated in interphase 3D-fixed nuclei by means of FISH and high-resolution cytometry. Changes of the structure re latively to the normal tissue xill be compared with changes of gene expression. Conclusion will be made on the relationship between the structure of chromosomes and expression of genes during cancerogenesis. It may give us new and relatively cheap method for the detection of oncogenic transformation in the specific tissue. The project will bring DNA-microarrays to the second laboratory in the Czech Republic, which will be important for the regional development of progressive tehnologies.
Publications
Total number of publications: 18
2004
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Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Year: 2004, type: Conference abstract
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Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium
Chromosoma, year: 2004, volume: 112, edition: 5
2003
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Cytogenetics and cytology of retinoblastomas
J Cancer Res. Clin.Oncol., year: 2003, volume: 129, edition: 2
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Nuclear and territorial topography of chromosome telomeres in human lymphocytes
Experimental Cell Research, year: 2003, volume: 289, edition: 1
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Study of the gene expression in malignant diseases by cDNA microarray technology
Biophysics of the Genome, year: 2003
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Topography of telomeres and structure of chromosome territories in human lymphocytes
Biophysics of the Genome, year: 2003
2002
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Nuclear topography and gene activity
Biophysics of the genome and its interactions, year: 2002
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Structure of the human genome: Experimental results and models
Biophysics of the genome and its interactions, year: 2002