Project information
Modulatory role of sigma signalling in electromechanical coupling of isolated cardiomyocyte and heart
- Project Identification
- GA305/04/1385
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Medicine
- prof. MUDr. Marie Nováková, Ph.D.
- prof. MUDr. Drahomír Horký, DrSc.
- MUDr. Lenka Krejčířová, Ph.D.
- doc. Ing. Michal Pásek, Ph.D.
- Keywords
- sigma receptor, membrane surrents, cardiac contrctility
- Cooperating Organization
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Brno University of Technology
- Responsible person Prof.,Ing. Ivo Provazník, Ph.D.
- Responsible person jana Bardoňová
The proposed project deals with the role of sigma receptors in mammalian myocardium. The experiments are designed to answer some elementary questions concerning the effect of sigma signallingt on electromechanical coupling in rat, guinea pig and rabbit heart. Since the effect of sigma receptor binding has been previously studied mainly in rat ventricular cardiomyocytes, it is necessary to extend the experiments also to isolated heart and multicellular heart preparation and to other species. The project is focused on detailed analysis of electrical and mechanical consequences of sigma receptor binding in mammalian myocardium, with respect to differences between species and differences between preparations used (isolated cardiomyocytes, multicellular heart preparations, isolted heart). To study electrophysiological effects of sigma ligands in heart muscle voltage clamp and patch clamp techniqes will be used. Moreover, recording of monophasic action potentials by voltage sensitive dyes will be employed. Mechanical properties of cardiac muscle will be studied by analyses of left ventricular pressure curves and mechanograms from multicellular heart preparations. Putative cytotoxic effects of sigma ligands will be studied in morphological part of the project using electron microschopy techniques. The expected contribution of this project is obtaining of the electrophysiological correlates to inotropic action of sigma receptors and mapping differences between species.
Publications
Total number of publications: 30
2006
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Statistical Analysis in Complex-Valued Wavelet Detection of Acute Myocardial Ischemia in Rabbit
Biomedical Engineering Conference, year: 2006
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The Effect Of Perphenazine On Transient Outward Potassium Current In Rat Ventricular Myocytes
Analysis of Biomedical Signals and Images, year: 2006
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The effect of perphenazine on transient outward potassium current in rat ventricular myocytes
Analysis of Biomedical Signals and Images, year: 2006, volume: 18, edition: 1
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The effect of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit isolated heart
France - New EU members meeting: New frontiers in basic cardiovascular research:Subcellular mechanisms of altered muscle function in cardiovascular diseases, year: 2006
2005
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Action potential voltage clamp analysis of rate-dependent changes of action potential in rat ventricular myocytes
Year: 2005, type: Conference abstract
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Analysis of dynamic changes in ECG signals during optical mapping by dynamic time warping
Computers in Cardiology, year: 2005
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Analýza frekvenčně závislých změn průběhu akčního napětí metodou vnuceného akčního napětí u komorových srdečních buněk potkana
Zborník prác a abstraktov prezentovaných na 81. Fyziologických dňoch, year: 2005
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Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models
Computers in Cardiology, year: 2005
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ECG segment variability during voltage-sensitive dye application in animal heart
Scripta medica, year: 2005, volume: 78, edition: 5
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Effect of Sigma Ligand Haloperidol on Transient Outward Potassium Current in Rat Cardiomyocytes
Journal of Molecular and Cellular Cardiology, year: 2005, volume: 39, edition: 1