Project information
Modulatory role of sigma signalling in electromechanical coupling of isolated cardiomyocyte and heart
- Project Identification
- GA305/04/1385
- Project Period
- 1/2004 - 12/2006
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Medicine
- prof. MUDr. Marie Nováková, Ph.D.
- prof. MUDr. Drahomír Horký, DrSc.
- MUDr. Lenka Krejčířová, Ph.D.
- doc. Ing. Michal Pásek, Ph.D.
- Keywords
- sigma receptor, membrane surrents, cardiac contrctility
- Cooperating Organization
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Brno University of Technology
- Responsible person Prof.,Ing. Ivo Provazník, Ph.D.
- Responsible person jana Bardoňová
The proposed project deals with the role of sigma receptors in mammalian myocardium. The experiments are designed to answer some elementary questions concerning the effect of sigma signallingt on electromechanical coupling in rat, guinea pig and rabbit heart. Since the effect of sigma receptor binding has been previously studied mainly in rat ventricular cardiomyocytes, it is necessary to extend the experiments also to isolated heart and multicellular heart preparation and to other species. The project is focused on detailed analysis of electrical and mechanical consequences of sigma receptor binding in mammalian myocardium, with respect to differences between species and differences between preparations used (isolated cardiomyocytes, multicellular heart preparations, isolted heart). To study electrophysiological effects of sigma ligands in heart muscle voltage clamp and patch clamp techniqes will be used. Moreover, recording of monophasic action potentials by voltage sensitive dyes will be employed. Mechanical properties of cardiac muscle will be studied by analyses of left ventricular pressure curves and mechanograms from multicellular heart preparations. Putative cytotoxic effects of sigma ligands will be studied in morphological part of the project using electron microschopy techniques. The expected contribution of this project is obtaining of the electrophysiological correlates to inotropic action of sigma receptors and mapping differences between species.
Publications
Total number of publications: 30
2005
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Effect of sigma receptor ligand haloperidol on cardiac excitability
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Effects of Voltage Sensitive Dye di-4-ANEPPS on Guinea Pig Myocardium
IFMBE Proceedings, EMBEC 05, year: 2005
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Interaction of haloperidol with L-type voltage dependent calcium channels
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Interakcia haloperidolu s L-typom vápníkového kanála
Zborník prác a abstraktov prezentovaných na 81.Fyziologických doch, year: 2005
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Účinky sigma ligandu haloperidolu na membránové proudy kardiomyocytů potkana
Zborník prác a abstraktov prezentovaných na 81. Fyziologických dňoch, year: 2005
2004
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Acquisition and Pre-Processing Of Action Potentials Optically Recorded From Animal Heart
Analysis of Biomedical Signals and Images, year: 2004
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Efekt haloperidolu na srdcové napäťovo závislé iónové kanály
Tretie ivanské dni mladých biológov, year: 2004
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Effect of haloperidol on voltage dependent cardiac ion channels
Membrane Channels, Transporters and Receptors, year: 2004
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Effect of Sigma Ligand Haloperidol on Cardiac Excitability
New Frontiers in Basic Cardiovascular Research, year: 2004
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Effect of Sigma Receptor Ligand Haloperidol in Guinea Pig Heart
Acta Veterinaria Brunensis, year: 2004, volume: 73, edition: 3