Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2015043
- Project Period
- 1/2016 - 12/2019
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
-
Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec, Prague-West) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies (total investments will exceed 750 mil. CZK by the end of 2015), will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB as National Affiliated Center is a part of the ESFRI projects INSTRUCT.
Publications
Total number of publications: 312
2020
-
Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
Journal of Applied Crystallography, year: 2020, volume: 53, edition: DEC, DOI
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Direct fluorogenic detection of palladium and platinum organometallic complexes with proteins and nucleic acids in polyacrylamide gels
Scientific reports, year: 2020, volume: 10, edition: 1, DOI
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DSS1 interacts with and stimulates RAD52 to promote the repair of DSBs
Nucleic Acids Research, year: 2020, volume: 48, edition: 2, DOI
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Electrochemical determination of insulin at CuNPs/chitosan-MWCNTs and CoNPs/chitosan-MWCNTs modified screen printed carbon electrodes
Journal of Electroanalytical Chemistry, year: 2020, volume: 860, edition: MAR 1 2020, DOI
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Encapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles
Nature Communications, year: 2020, volume: 11, edition: 1, DOI
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Heterometallic 3d-4f {Co2Gd4} phosphonates: new members of the potential magnetic cooler family
New Journal of Chemistry, year: 2020, volume: 44, edition: 2, DOI
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Highly porous hybrid metallosilicate materials prepared by non-hydrolytic sol-gel: Hydrothermal stability and catalytic properties in ethanol dehydration
Microporous and Mesoporous Materials, year: 2020, volume: 297, edition: MAY 1 2020, DOI
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HSPA1A conformational mutants reveal a conserved structural unit in Hsp70 proteins
Biochimica et Biophysica Acta - General Subjects, year: 2020, volume: 1864, edition: 1, DOI
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Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold
International journal of biological macromolecules, year: 2020, volume: 152, edition: JUN, DOI
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Isoforms of Cathepsin B1 in Neurotropic Schistosomula of Trichobilharzia regenti Differ in Substrate Preferences and a Highly Expressed Catalytically Inactive Paralog Binds Cystatin
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, year: 2020, volume: 10, edition: FEB, DOI