Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2015043
- Project Period
- 1/2016 - 12/2019
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
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Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec, Prague-West) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies (total investments will exceed 750 mil. CZK by the end of 2015), will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB as National Affiliated Center is a part of the ESFRI projects INSTRUCT.
Publications
Total number of publications: 312
2019
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Comparative structural analysis of lectin family from Photorhabdus spp.
Year: 2019, type: Conference abstract
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Days of Electron Microscopy 2019
Year: 2019, type: Popularization text
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DDA, DIA, či directDIA: závisle, s knihovnou či bez? Praktické porovnání přístupů pro různé typy proteomických experimentů
Year: 2019, type: Appeared in Conference without Proceedings
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Deconstruction of microfibrillated cellulose into nanocrystalline cellulose rods and mesogenic phase formation in concentrated low-modulus sodium silicate solutions
Cellulose, year: 2019, volume: 26, edition: 7, DOI
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Different Modes of Action of Genetic and Chemical Downregulation of Histone Deacetylases with Respect to Plant Development and Histone Modifications
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, year: 2019, volume: 20, edition: 20, DOI
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Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
Nature Communications, year: 2019, volume: 10, edition: APR 18 2019, DOI
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DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1 beta-Ser88
CELLS, year: 2019, volume: 8, edition: 9, DOI
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Efficient and robust preparation of tyrosine phosphorylated intrinsically disordered proteins
BioTechniques, year: 2019, volume: 67, edition: 1, DOI
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Enterovirus particles expel capsid pentamers to enable genome release
Nature Communications, year: 2019, volume: 10, edition: MAR, DOI
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Experimental Investigation of Thermal Properties of Binary Metal Alloys
Year: 2019, type: Appeared in Conference without Proceedings