Project information

Structural studies of protein-RNA complexes involved in RNA quality control

Project Identification
Project Period
1/2008 - 12/2010
Investor / Pogramme / Project type
Ministry of Education, Youth and Sports of the CR
MU Faculty or unit
Faculty of Science
RNA quality control; misfolded RNA; RNA turnover; RNA recognition; protein-RNA complexes; structure; nuclear magnetic resonance

Cells seem to leave nothing to chance, including the quality control at all steps of information transfer. RNA maturation involves extensive processing that has a relatively high error rate. Cells have developed an RNA quality control mechanism which identifies improperly processed RNAs and subsequently degrades them. This mechanism ensures that only properly processed RNAs are engaged in protein synthesis. The exosome and Trf4 complex are the central molecular machines in this process. The Trf4 complex recognizes and polyadenylates the aberrant RNAs, whereas the exosome is responsible for their degradation. We propose here to use multidimensional NMR spectroscopy to determine the three-dimensional structure of several protein-RNA complexes that are involved in RNA quality control. The proposed project will reveal the mechanism by which the Trf4 complex identifies the aberrant RNAs and thus recruit them for the degradation machinery.


Total number of publications: 10

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