Project information
Changes of the cytoskeleton in tumour cell lines during induced apoptosis in relation to different stages of differentiation
- Project Identification
- GA304/00/0403
- Project Period
- 1/2000 - 1/2002
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Medicine
Apoptosis, a programmed cell death, is worth of study not only as aform of physiological cell death but also as a promising approach to the casual theraphy of malignant diseases. In spite of great progress in this field is not well understood how the apo ptosis is modulated in differentiated cells and how cytoskeletal components are involved. In this project a detailed study of the cytoskeletal components in two tumour cell lines is proposed with the aim to define a role of the cytoskeleton in the trigge ring and the course of apoptosis. Human promyelocytic leukemia HL-60 and mouse embryonal carcinoma P19 cell lines will be used as well as their differentiated forms-monocytes, granulocytes and macrophages (HL-60 cells) and neural, glial and muscle cells (P19 cells). The apoptosis will be triggered by chemical inductors including cytoskeletal toxins and different techniques will be applied to describe the process at the molecular (SDS-PAGE, immunoblotting), microscopical (immunofluorescence) and ultrastr
Publications
Total number of publications: 28
2001
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Vliv krátkovlnného UV záření na strukturu a životaschopnost diploidních a transformovaných lidských buněk in vitro ve vztahu k možnosti ovlivnění vybranými antioxidanty
Year: 2001, type: Conference abstract
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Vliv UV záření na strukturu a životaschopnost diploidních a transformovaných lidských buněk in vitro ve vztahu k možnosti ovlivnění vybranými antioxidanty
Year: 2001, type: Conference abstract
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Změny cytoskeletu během procesu apoptózy
XVI. Biologické dny - Aktuální témata z buněčné a molekulární biologie, year: 2001
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Změny cytoskeletu buněk linie HL-60 v průběhu apoptózy indukované kyselinou all-trans retinovou
Year: 2001, type: Conference abstract
2000
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Changes in cytoskeleton of HL-60 cells during induced apoptosis
Year: 2000, type: Conference abstract
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Vliv kyseliny retinové na cytoskeletalní strukturu buněk linie HL-60
Year: 2000, type: Conference abstract
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Změny aktinového cytoskeletu buněk promyelocytární leukemické linie HL-60 v průběhu diferenciace a apoptózy
Cells II, year: 2000
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Změny mikrotubulů buněk linie HL-60 v důsledku kombinovaného působení ultrazvuku a vybraných cytostatik
Year: 2000, type: Conference abstract