Publication details

Electrochemical detection of miR-34a-5p as a promising tool for HNC and PCa diagnosis

Authors

HUDCOVÁ Kristýna VEČEŘOVÁ Aneta TRNKOVÁ Libuše KEJNOVSKÁ Iva VORLÍČKOVÁ Michaela MASAŘÍK Michal

Year of publication 2015
Type Article in Proceedings
MU Faculty or unit

Central European Institute of Technology

Citation
Keywords MiRNAs; head and neck cancer; prostate cancer; PCR analysis; CD spectra; UV absorption spectra; voltammetric methods
Description MicroRNAs (miRNAs) are a class of non-coding RNAs that are intensively studied as biomarkers of various diseases. In this study, the attention was aimed at the research of miR-34a-5p related to head and neck cancer (HNC) and prostate cancer (PCa). MiR-34a-5p belongs to the miR-34 family and its directly linked to p53 and Wnt pathways. beside the classical molecular biology techniques of miRNA study (PCR, Northern Blotting, microarrays technologies), new biophysical approaches were applied. The results from the PCR analysis were complemented with UV absorption spectra, CD spectra and linear sweep voltammetry in connection with adsorptive stripping (AdTS) technique indicating significant differences in miR-34a-5p expression status.
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