Publication details

Advances in the cellular structural biology of nucleic acids

Authors

GIASSA Ilektra-Chara RYNEŠ Jan FESSL T. TRANTÍRKOVÁ Silvie TRANTÍREK Lukáš

Type Article in Periodical
Magazine / Source FEBS LETTERS
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.13054
Doi http://dx.doi.org/10.1002/1873-3468.13054
Keywords DNA; in-cell chemical probing; in-cell EPR; in-cell NMR; in-cell single-molecule FRET; RNA
Description Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.
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