Publication details

The depletion of p38alpha kinase upregulates NADPH oxidase 2/NOX2/gp91 expression and the production of superoxide in mouse embryonic stem cells

Authors

BINÓ Lucia VESELÁ Iva PAPEŽÍKOVÁ Iva PROCHÁZKOVÁ Jiřina VAŠÍČEK Ondřej ŠTEFKOVÁ Kateřina KUČERA Jan HANÁČKOVÁ Markéta KUBALA Lukáš PACHERNÍK Jiří

Year of publication 2019
Type Article in Periodical
Magazine / Source Archives of Biochemistry and Biophysics
MU Faculty or unit

Faculty of Science

Citation
Web https://www.sciencedirect.com/science/article/pii/S0003986118303813
Doi http://dx.doi.org/10.1016/j.abb.2019.06.001
Keywords Embryonic stem cell; p38 kinase; Reactive oxygen species; NADPH oxidase
Description P38alpha kinase plays an important role in the regulation of both cell stress response and cell fate. In this study, we report that p38alpha kinase-deficient embryonic stem cells exhibit a higher production of reactive oxygen species (ROS) in contrast to their wild-type counterpart. Analysis of the expressions of NADPH oxidases (NOXs) and dual oxidases, crucial enzymes involved in intracellular ROS formation, shows NOX2/gp91phox is over-expressed in p38alpha deficient cells. The particular increase in superoxide formation was confirmed by the specific detection of hydroethidine derivate 2-hydroxyethidium. ROS formation decreased when the level of NOX2 was silenced by siRNA in p38alpha deficient cells. These data suggest the importance of p38alpha kinase in the regulation of ROS metabolism in embryonic stem cells and the significance of the observed phenomena of cancer cell-like phenotypes, which is discussed.
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